Kempner function

Template:Chembox para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays. Under their influence the decay to yellow para-nitrophenol is catalysed. This product can be measured with a 405 nm spectrophotometer.

$PNPP+H_{2}O\;{\overrightarrow {_{AP}}}\;PNP+P_{i}$

External links

• pNPP disodium salt hexahydrate (SigmaAldrich.com)
• Nunc Brand Products

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