Symmetric function

The Purnell equation is an equation used in analytical chemistry to calculate the resolution R_s between two peaks in a chromatogram:^[1]^[2]^[3]

$R_{s}={\frac {\sqrt {N_{2}}}{4}}\left({\frac {\alpha -1}{\alpha }}\right)\left({\frac {k'_{2}}{1+k'_{2}}}\right)$

where

R_s is the resolution between the two peaks

N₂ is the plate number of the second peak

α is the separation factor between the two peaks

k '₂ is the retention factor of the second peak.

The higher the resolution, the better the separation.

References

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↑ Miller, J. M., Chromatography: Concepts and Contrasts, second edition, John Wiley & Sons, Inc., Hoboken, NJ, USA, 2005.
↑ Dean, J. R. et al., Practical Skills in Chemistry, first edition, Pearson Education Ltd., Harlow, UK, 2002, p. 205–224.
↑ C. Legido-Quigley, HPLC theory

[1] Miller, J. M., Chromatography: Concepts and Contrasts, second edition, John Wiley & Sons, Inc., Hoboken, NJ, USA, 2005.

[2] Dean, J. R. et al., Practical Skills in Chemistry, first edition, Pearson Education Ltd., Harlow, UK, 2002, p. 205–224.

[3] C. Legido-Quigley, HPLC theory

[1]

[2]

[3]

Symmetric function

References

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