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| The '''Purnell equation''' is an [[equation]] used in [[analytical chemistry]] to calculate the resolution ''R''<sub>s</sub> between two peaks in a [[Chromatogram#Chromatography_terms|chromatogram]]:<ref>Miller, J. M., ''Chromatography: Concepts and Contrasts'', second edition, John Wiley & Sons, Inc., Hoboken, NJ, USA, 2005.</ref><ref>Dean, J. R. et al., ''Practical Skills in Chemistry'', first edition, Pearson Education Ltd., Harlow, UK, 2002, p. 205–224.</ref><ref>[http://www.cristinalegido-quigley.co.uk/files/LECTURE%202,%20FUNDAMENTALS%20HPLC.pdf C. Legido-Quigley, ''HPLC theory'']</ref>
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| <math>R_s = \frac{\sqrt{N_2}}{4} \left ( \frac{\alpha - 1}{\alpha} \right ) \left ( \frac{k'_2}{1 + k'_2} \right )</math>
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| where
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| ::''R''<sub>s</sub> is the resolution between the two peaks
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| ::''N''<sub>2</sub> is the [[plate number]] of the second peak
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| ::''α'' is the separation factor between the two peaks
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| ::''k'' '<sub>2</sub> is the [[retention factor]] of the second peak.
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| The higher the resolution, the better the separation.
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| ==References==
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| {{reflist}}
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| [[Category:Chromatography]]
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| [[Category:Equations]]
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| {{analytical-chem-stub}}
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Latest revision as of 21:40, 14 December 2014
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