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| {{DISPLAYTITLE:''hisB''}}<!--lowercase first letter and italics-->
| | Hi there, I am Felicidad Oquendo. Delaware is our beginning location. Interviewing is what I do in my working day occupation. The thing I adore most flower arranging and now I have time to consider on new issues.<br><br>Also visit my weblog: [http://Dubaitoursrus.com/UserProfile/tabid/61/userId/122235/Default.aspx http://Dubaitoursrus.com/] |
| The '''hisB''' gene, found in the [[Enterobacteriaceae|enterobacteria]] (such as ''[[Escherichia coli|E. coli]]''), in ''[[Campylobacter jejuni]]'' and in ''[[Xylella]]''/''[[Xanthomonas]]'' encodes a protein involved in catalysis of two step in [[histidine biosynthesis]] (the sixth and eight step), namely the bifunctional [[Imidazoleglycerol-phosphate dehydratase]]/[[histidinol-phosphatase]].<ref>{{cite pmid|15042344}}</ref>
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| The former function ({{EC number|4.2.1.19}}), found at the [[N-terminal]], dehydrated <small>d</small>-erythroimidazoleglycerolphosphate to imidazoleacetolphosphate, the latter function ({{EC number|3.1.3.15}}), found at the [[C-terminal]], dephosphorylates <small>l</small>-histidinolphosphate producing histidinol.<ref>Parker95: Parker, A.R., Moore, J.A., Schwab, J.M., Davisson, V.J. (1995). "Escherichia coli Imidazoleglycerol Phosphate Dehydratase: Spectroscopic Characterization of the Enzymic Product and the Steric Course of the Reaction." Journal of the American Chemical Society.</ref><ref>{{cite pmid|6284708}}</ref><ref>{{cite pmid|6309747}}</ref> | |
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| The firth step is catalysed instead by histadinolphosphate aminotransferase (encoded by ''hisC'')<ref>{{cite pmid|21097882}}</ref>
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| The peptide is 40.5kDa and associates to form a hexamer (unless truncated)<ref>{{cite pmid|16966333}}</ref>
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| In E. coli hisB is found on the hisGDCBHAFI operon<ref>{{cite pmid|1592835}}</ref>
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| The phosphatase activity possess a substrate ambiguity and overexpression of hisB can rescue phosphoserine phosphatase (serB) knockouts.<ref>{{cite pmid|17884825}}</ref>
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| == Reactions ==
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| hisB-N
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| :D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate <math>\rightleftharpoons</math> 3-(imidazol-4-yl)-2-oxopropyl phosphate + H<sub>2</sub>O | |
| hisB-C
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| :L-histidinol phosphate + H<sub>2</sub>O <math>\rightleftharpoons</math> L-histidinol + phosphate
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| == Non-fusion protein in other species ==
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| [[HIS3]] from ''Saccharomyces cerevisiae'' is not a fused IGP dehydratase and hisidinol phosphatase, but an IGPD only (homologous to hisB-N). Whereas HIS2 is the HP (analogous to hisB-C, called hisJ in some prokaryotes).
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| == References ==
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| {{reflist}}
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| [[Category:Genes]]
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| {{genetics-stub}}
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Hi there, I am Felicidad Oquendo. Delaware is our beginning location. Interviewing is what I do in my working day occupation. The thing I adore most flower arranging and now I have time to consider on new issues.
Also visit my weblog: http://Dubaitoursrus.com/