View source for Cobalt chelatase

{{enzyme
| Name = dihydrofolate synthase
| EC_number = 6.3.2.12
| CAS_number = 37318-62-0
| IUBMB_EC_number = 6/3/2/12
| GO_code = 0008841
| image = 
| width = 
| caption = 
}}
In [[enzymology]], a '''dihydrofolate synthase''' ({{EC number|6.3.2.12}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]

:ATP + 7,8-dihydropteroate + L-glutamate <math>\rightleftharpoons</math> ADP + phosphate + 7,8-dihydropteroylglutamate

The 3 [[substrate (biochemistry)|substrates]] of this enzyme are [[adenosine triphosphate|ATP]], [[7,8-dihydropteroate]], and [[L-glutamate]], whereas its 3 [[product (chemistry)|products]] are [[adenosine diphosphate|ADP]], [[phosphate]], and [[7,8-dihydropteroylglutamate]].

This enzyme belongs to the family of [[ligase]]s, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases).  The systematic name of this enzyme class is '''7,8-dihydropteroate:L-glutamate ligase (ADP-forming)'''. Other names in common use include '''dihydrofolate synthetase''', '''7,8-dihydrofolate synthetase''', '''H2-folate synthetase''', '''7,8-dihydropteroate:L-glutamate ligase (ADP)''', '''dihydrofolate synthetase-folylpolyglutamate synthetase''', '''folylpoly-(gamma-glutamate) synthetase-dihydrofolate synthase''', '''FHFS''', '''FHFS/FPGS''', '''dihydropteroate:L-glutamate ligase (ADP-forming)''', and '''DHFS'''.  This enzyme participates in [[folate biosynthesis]].  

==Structural studies==

As of late 2007, 3 [[tertiary structure|structures]] have been solved for this class of enzymes, with [[Protein Data Bank|PDB]] accession codes {{PDB link|1W78}}, {{PDB link|1W7K}}, and {{PDB link|2BMB}}.

==References==
{{reflist|1}}
* {{cite journal | author = GRIFFIN MJ, BROWN GM | date = 1964 | title = THE BIOSYNTHESIS OF FOLIC ACID. III. ENZYMATIC FORMATION OF DIHYDROFOLIC ACID FROM DIHYDROPTEROIC ACID AND OF TETRAHYDROPTEROYLPOLYGLUTAMIC ACID COMPOUNDS FROM TETRAHYDROFOLIC ACID | journal = J. Biol. Chem.  | volume = 239 | pages = 310&ndash;6  | pmid = 14114858 }}
* {{cite journal | author = Bognar AL, Osborne C, Shane B, Singer SC, Ferone R | date = 1985 | title = Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product | journal = J. Biol. Chem.  | volume = 260 | pages = 5625&ndash;30  | pmid = 2985605 | issue = 9 }}
* {{cite journal | author = R, Rebeille F | date = 2001 | title = Tetrahydrofolate biosynthesis in plants: molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana | journal = Proc. Natl. Acad. Sci. U.S.A.  | volume = 98 | pages = 15360&ndash;5  | pmid = 11752472 | doi = 10.1073/pnas.261585098 | last2 = Cherest | first2 = H | last3 = Jabrin | first3 = S | last4 = Grunwald | first4 = D | last5 = Surdin-Kerjan | first5 = Y | last6 = Douce | first6 = R | last7 = Rébeillé | first7 = F | issue = 26 | pmc = 65034 }}
* {{cite journal | author = Cherest H, Thomas D, Surdin-Kerjan Y | date = 2000 | title = Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae | journal = J. Biol. Chem.  | volume = 275 | pages = 14056&ndash;63  | pmid = 10799479 | doi = 10.1074/jbc.275.19.14056 | issue = 19 }}
* {{cite journal | author = Cossins EA, Chen L | date = 1997 | title = Folates and one-carbon metabolism in plants and fungi | journal = [[Phytochemistry (journal)|Phytochemistry]].  | volume = 45 | pages = 437&ndash;52  | pmid = 9190084 | doi = 10.1016/S0031-9422(96)00833-3 | issue = 3 }}

[[Category:EC 6.3.2]]
[[Category:Enzymes of known structure]]

{{ligase-stub}}