View source for ADP-dependent medium-chain-acyl-CoA hydrolase

{{enzyme
| Name = aminoacyl-tRNA hydrolase
| EC_number = 3.1.1.29
| CAS_number = 9054-98-2
| IUBMB_EC_number = 3/1/1/29
| GO_code = 0004045
| image = 
| width = 
| caption = 
}}
In [[enzymology]], an '''aminoacyl-tRNA hydrolase''' ({{EC number|3.1.1.29}}) is an [[enzyme]] that [[catalysis|catalyzes]] the [[chemical reaction]]

:N-Substituted aminoacyl-tRNA + H<sub>2</sub>O <math>\rightleftharpoons</math> N-substituted amino acid + tRNA

Thus, the two [[substrate (biochemistry)|substrates]] of this enzyme are [[N-Substituted aminoacyl-tRNA]] and [[water|H<sub>2</sub>O]], whereas its two [[product (chemistry)|products]] are [[N-substituted amino acid]] and [[tRNA]].

This enzyme belongs to the family of [[hydrolase]]s, specifically those acting on carboxylic [[ester]] bonds.  The systematic name of this enzyme class is '''aminoacyl-tRNA aminoacylhydrolase'''. Other names in common use include '''aminoacyl-transfer ribonucleate hydrolase''', '''N-substituted aminoacyl transfer RNA hydrolase''', and '''peptidyl-tRNA hydrolase'''.  

==Structural studies==

As of late 2007, 9 [[tertiary structure|structures]] have been solved for this class of enzymes, with [[Protein Data Bank|PDB]] accession codes {{PDB link|1RYB}}, {{PDB link|1WN2}}, {{PDB link|1XTY}}, {{PDB link|2D3K}}, {{PDB link|2E1B}}, {{PDB link|2PTH}}, {{PDB link|2Z2I}}, {{PDB link|2Z2J}}, and {{PDB link|2Z2K}}.

==References==
{{reflist|1}}
* {{cite journal | author = Jost JP, Bock RM | date = 1969 | title = Enzymatic hydrolysis of N-substituted aminoacyl transfer ribonucleic acid in yeast | journal = J. Biol. Chem.  | volume = 244 | pages = 5866&ndash;73  | pmid = 4981785 | issue = 21 }}

{{hydrolase-stub}}

[[Category:EC 3.1.1]]
[[Category:Enzymes of known structure]]